raw single-cell rna-seq data drop-seq  (10X Genomics)

Journal: eLife

Article Title: FBXO24 deletion causes abnormal accumulation of membraneless electron-dense granules in sperm flagella and male infertility

doi: 10.7554/eLife.92794

Figure Lengend Snippet: ( A ) Expression profile of mouse Fbxo24 in spermatogenic cells is shown. Expression profiling was obtained from the testis single-cell RNA-seq dataset . Ud spg: undifferentiated spermatogonia, A1- A2 Spg: A1 and A2 differentiating spermatogonia, A3-B Spg: A3, A4, In, and B differentiating spermatogonia, Prele Sc: preleptotene spermatocytes, Le/Zy Sc: leptotene/zygotene spermatocytes, Pa Sc: pachytene spermatocytes, Di/Se Sc: diplotene/secondary spermatocytes, Early St: early round spermatids, Mid St: mid round spermatids, Late St: late round spermatids, SC: Sertoli cells, PTM: peritubular myoid cells, LC: Leydig cells, and EC: endothelial cells. ( B ) Expression of human FBXO24 in spermatogenic cells is shown. Expression profiling was obtained from the testis single-cell RNA-seq dataset . Dif Spg: differentiating spermatogonia. ( C ) Pairwise sequence alignment of amino acids between mouse and human FBXO24. Dark blue highlight indicates identical amino acids. ( D ) The F-box domain of mouse and human FBXO24 was identified with a protein database search (Simple Modular Architecture Research Tool [SMART]; http://smart.embl-heidelberg.de/ ).

Article Snippet: The following previously published dataset was used: Hermann BP Cheng K Singh A Roa-De La Cruz L Mutoji KN Chen IC Gildersleeve H Lehle JD Mayo M Westernströer B Law NC Oatley MJ Velte EK Niedenberger BA Fritze D Silber S Geyer CB Oatley JM McCarrey JR 2018 Queryable single-cell RNA-seq (Drop-Seq) datasets of Human and Mouse spermatogenic cells Mendeley Data 10.17632/kxd5f8vpt4.1

Techniques: Expressing, RNA Sequencing, Sequencing

Journal: eLife

Article Title: FBXO24 deletion causes abnormal accumulation of membraneless electron-dense granules in sperm flagella and male infertility

doi: 10.7554/eLife.92794

Figure Lengend Snippet: Spermatozoa were obtained from Fbxo24 heterozygous or knockout (KO) male mice. Immature spermatogenic cells or somatic cells were rarely observed.

Article Snippet: The following previously published dataset was used: Hermann BP Cheng K Singh A Roa-De La Cruz L Mutoji KN Chen IC Gildersleeve H Lehle JD Mayo M Westernströer B Law NC Oatley MJ Velte EK Niedenberger BA Fritze D Silber S Geyer CB Oatley JM McCarrey JR 2018 Queryable single-cell RNA-seq (Drop-Seq) datasets of Human and Mouse spermatogenic cells Mendeley Data 10.17632/kxd5f8vpt4.1

Techniques: Knock-Out